Bloodborne Wiki

Some materials you will need to farm to access every chalice dungeon. Share your best farming glyph for that specific material in the designated box. These should all be in a layer 1 if possible, though not all are.

Best Farming Glyph Per Material
Item Quanitity Glyph Layer Chalice Where in your Glyph:
Ritual Blood (1)
Ritual Blood (2)
Ritual Blood (3)
Ritual Blood (4)
Ritual Blood (5) 3 wmbrffjr 1 Open the door at the first lantern, two in a large chest directly ahead. Another in a smaller chest down the hall by werewolves
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Tomb Mold (1)
Tomb Mold (2)
Tomb Mold (3)
Tomb Mold (4)
Tomb Mold (5)
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Coldblood Flower Bulb
Blooming Coldblood Flower
Bloodshot Eyeball 8 exb8uivs 2 Lower Hintertomb
Living String N/A N/A N/A Obtained from killing the Brain of Mensis in Nightmare Frontier

or purchasing from a insight vendor at the cost of 10 insight

Red Jelly **Also can be found in the second floor lecture hall in a chest
Pearl Slug
Sage's Wrist 3 exb8uivs 3 Lower Hintertomb
Sage's Hair 6 huqfu4w

(Does not work)

3 Sinister Isz
Inflicted Organ
Yellow Backbone 3 Defeat The One Reborn for 3

Purchase from Insight vendor in Dream.

Bastard of Loran 2 and 3